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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
14/04/2009 |
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Data da última atualização: |
30/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COELHO, M. R. R.; MARRIEL, I. E.; JENKINS, S. N.; LANYON, C. V.; SELDIN, L.; O'DONNELL, A. G. |
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Afiliação: |
Márcia R. R. Coelho, UFRJ; IVANILDO EVODIO MARRIEL, CNPMS; Sasha N. Jenkins, Newcastle University; Clare V. Lanyon, Newcastle University; Lucy Seldin, UFRJ; Anthony G. O'Donnell, Newcastle University. |
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Título: |
Molecular detection and quantification of nifH gene sequences in the rhizosphere of sorghum (Sorghum bicolor) sown with two levels of nitrogen fertilizer. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Applied Soil Ecology, Amsterdam, v. 42, n. 1, p. 48-53, 2009. |
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DOI: |
10.1016/j.apsoil.2009.01.010 |
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Idioma: |
Inglês |
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Conteúdo: |
Denaturing gradient gel electrophoresis (DGGE) and SYBR Green I quantitative real-time PCR (qPCR) approaches were used to assess respectively the molecular diversity and the quantity of the nifH gene sequences in the rhizospheres of two cultivars of sorghum sown in Cerrado soil with contrasting levels of nitrogen fertilizer. DGGE fingerprinting showed that for both cultivars the presumptive nitrogen-fixing populations in the rhizospheres were more diverse than in bulk soil. Sequencing of nifH gene fragments obtained from DGGE bands revealed that members of the order Rhizobiales were prevalent among the dominant diazotrophs. Discriminant analysis of DGGE profiles resulted into three groups formed by (i) cultivar BRS 308 sown with high level of nitrogen, (ii) cultivar BRS 308 sown with low level of nitrogen and cultivar BRS 310 sown either with low or high levels of nitrogen and (iii) bulk soil, showing that the nitrogen fertilization influenced the nifH gene sequence diversity only in the rhizosphere of cultivar BRS 308. When the quantity of the nifH gene sequences was determined by q-PCR, 2.4 x105 to 1.3 x 107 copies/g of soil were detected. The highest number of nifH gene copies was observed in the rhizosphere of cultivar BRS 308 treated with low amount of fertilizer, and a reduction in nifH density was observed in the rhizospheres of both sorghum cultivars when high levels of nitrogen were applied. Thus, both the amount of nitrogen fertilizer and the cultivar are important factors influencing the structure and amount of diazotrophs in sorghum. MenosDenaturing gradient gel electrophoresis (DGGE) and SYBR Green I quantitative real-time PCR (qPCR) approaches were used to assess respectively the molecular diversity and the quantity of the nifH gene sequences in the rhizospheres of two cultivars of sorghum sown in Cerrado soil with contrasting levels of nitrogen fertilizer. DGGE fingerprinting showed that for both cultivars the presumptive nitrogen-fixing populations in the rhizospheres were more diverse than in bulk soil. Sequencing of nifH gene fragments obtained from DGGE bands revealed that members of the order Rhizobiales were prevalent among the dominant diazotrophs. Discriminant analysis of DGGE profiles resulted into three groups formed by (i) cultivar BRS 308 sown with high level of nitrogen, (ii) cultivar BRS 308 sown with low level of nitrogen and cultivar BRS 310 sown either with low or high levels of nitrogen and (iii) bulk soil, showing that the nitrogen fertilization influenced the nifH gene sequence diversity only in the rhizosphere of cultivar BRS 308. When the quantity of the nifH gene sequences was determined by q-PCR, 2.4 x105 to 1.3 x 107 copies/g of soil were detected. The highest number of nifH gene copies was observed in the rhizosphere of cultivar BRS 308 treated with low amount of fertilizer, and a reduction in nifH density was observed in the rhizospheres of both sorghum cultivars when high levels of nitrogen were applied. Thus, both the amount of nitrogen fertilizer and the cultivar are important... Mostrar Tudo |
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Thesagro: |
Sorgo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53597/1/Molecular-detection.pdf
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Marc: |
LEADER 02260naa a2200205 a 4500
001 1491948
005 2018-05-30
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1016/j.apsoil.2009.01.010$2DOI
100 1 $aCOELHO, M. R. R.
245 $aMolecular detection and quantification of nifH gene sequences in the rhizosphere of sorghum (Sorghum bicolor) sown with two levels of nitrogen fertilizer.$h[electronic resource]
260 $c2009
520 $aDenaturing gradient gel electrophoresis (DGGE) and SYBR Green I quantitative real-time PCR (qPCR) approaches were used to assess respectively the molecular diversity and the quantity of the nifH gene sequences in the rhizospheres of two cultivars of sorghum sown in Cerrado soil with contrasting levels of nitrogen fertilizer. DGGE fingerprinting showed that for both cultivars the presumptive nitrogen-fixing populations in the rhizospheres were more diverse than in bulk soil. Sequencing of nifH gene fragments obtained from DGGE bands revealed that members of the order Rhizobiales were prevalent among the dominant diazotrophs. Discriminant analysis of DGGE profiles resulted into three groups formed by (i) cultivar BRS 308 sown with high level of nitrogen, (ii) cultivar BRS 308 sown with low level of nitrogen and cultivar BRS 310 sown either with low or high levels of nitrogen and (iii) bulk soil, showing that the nitrogen fertilization influenced the nifH gene sequence diversity only in the rhizosphere of cultivar BRS 308. When the quantity of the nifH gene sequences was determined by q-PCR, 2.4 x105 to 1.3 x 107 copies/g of soil were detected. The highest number of nifH gene copies was observed in the rhizosphere of cultivar BRS 308 treated with low amount of fertilizer, and a reduction in nifH density was observed in the rhizospheres of both sorghum cultivars when high levels of nitrogen were applied. Thus, both the amount of nitrogen fertilizer and the cultivar are important factors influencing the structure and amount of diazotrophs in sorghum.
650 $aSorgo
700 1 $aMARRIEL, I. E.
700 1 $aJENKINS, S. N.
700 1 $aLANYON, C. V.
700 1 $aSELDIN, L.
700 1 $aO'DONNELL, A. G.
773 $tApplied Soil Ecology, Amsterdam$gv. 42, n. 1, p. 48-53, 2009.
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Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
15/10/2019 |
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Data da última atualização: |
16/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SCHEPASCHENKO, D.; CHAVE, J.; PHILLIPS, O. L.; LEWIS, S. L.; DAVIES, S. J.; RÉJOU-MÉCHAIN, M.; SIST, P.; SCIPAL, K.; PERGER, C.; HERAULT, B.; LABRIÈRE, N.; HOFHANSL, F.; AFFUM-BAFFOE, K.; ALEINIKOV, A.; ALONSO, A.; AMANI, C.; ARAUJO-MURAKAMI, A.; ARMSTON, J.; ARROYO, L.; ASCARRUNZ, N.; AZEVEDO, C. P. de; BAKER, T.; BALAZY, R.; BEDEAU, C.; BERRY, N.; BILOUS, A. M.; BILOUS, S. Y.; BISSIENGOU, P.; BLANC, L.; BOBKOVA, K. S.; BRASLAVSKAYA, T.; BRIENEN, R.; BURSLEM, D. F. R. P.; CONDIT, R.; CUNI-SANCHEZ, A.; DANILINA, D.; TORRES, D. del C.; DERROIRE, G.; DESCROIX, L.; SOTTA, E. D.; OLIVEIRA, M. V. N. d'; DRESEL, C.; ERWIN, T.; EVDOKIMENKO, M. D.; FALCK, J.; FELDPAUSCH, T. R.; FOLI, E. G.; FOSTER, R.; FRITZ, S.; GARCIA-ABRIL, A. D.; GORNOV, A.; GORNOVA, M.; GOTHARD-BASSÉBÉ, E.; GOURLET-FLEURY, S.; GUEDES, M. C.; HAMER, K. C.; SUSANTY, F. H.; HIGUCHI, N.; CORONADO, E. N. H.; HUBAU, W.; HUBBELL, S.; ILSTEDT, U.; IVANOV, V. V.; KANASHIRO, M.; KARLSSON, A.; KARMINOV, V. N.; KILLEEN, T.; KOFFI, J. C. K.; KONOVALOVA, M.; KRAXNER, F.; KREJZA, J.; KRISNAWATI, H.; KRIVOBOKOV, L. V.; KUZNETSOV, M. A.; LAKYDA, I.; LAKYDA, P. I.; LICONA, J. C.; LUCAS, R. M.; LUKINA, N.; LUSSETTI, D.; MALHI, Y.; MANZANERA, J. A.; MARIMON, B.; MARIMON JUNIOR, B. H.; VASQUEZ MARTINEZ, R.; MARTYNENKO, O. V.; MATSALA, M.; MATYASHUK, R. K.; FREITAS, L. J. M. de; MEMIAGHE, H.; MENDOZA, C.; MONTEAGUDO MENDONZA, A.; MOROZIUK, O. V.; MUKHORTOVA, L.; MUSA, S.; NAZIMOVA, D. I.; OKUDA, T.; OLIVEIRA, L. C. de; ONTIKOV, P. V.; OSIPOV, A. F.; PIETSCH, S.; PLAYFAIR, M.; POULSEN, J.; RADCHENKO, V. G.; RODNEY, K.; ROZAK, A. H.; RUSCHEL, A. R.; RUTISHAUSER, E.; SEE, L.; SHCHEPASHCHENKO, M.; SHEVCHENKO, N.; SHVIDENKO, A.; SILVEIRA, M.; SINGH, J.; SONKÉ, B.; SOUZA, C. R. de; STERENCZAK, K.; STONOZHENKO, L.; SULLIVAN, M. J. P.; SZATNIEWSKA, J.; TAEDOUMG, H.; TER STEEGE, H.; TIKHONOVA, E.; TOLEDO, M.; TREFILOVA, O. V.; VALBUENA, R.; VALENZUELA GAMARRA, L.; VASILIEV, S.; VEDROVA, E. F.; VERHOVETS, S. V.; VIDAL, E.; VLADIMIROVA, N. A.; VLEMINCKX, J.; VOS, V. A.; VOZMITEL, F. K.; WANEK, W.; WEST, T. A. P.; WOELL, H.; WOODS, J. T.; WORTEL, V.; YAMADA, T.; HAJAR, Z. S. N.; ZO-BI, I. C. |
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Afiliação: |
DMITRY SCHEPASCHENKO; JÉRÔME CHAVE; OLIVER L. PHILLIPS; SIMON L. LEWIS; STUART J. DAVIES; MAXIME RÉJOU-MÉCHAIN; PLINIO SIST; KLAUS SCIPAL; CHRISTOPH PERGER; BRUNO HERAULT; NICOLAS LABRIÈRE; FLORIAN HOFHANSL; KOFI AFFUM-BAFFOE; ALEXEI ALEINIKOV; ALFONSO ALONSO; CHRISTIAN AMANI; ALEJANDRO ARAUJO-MURAKAMI; JOHN ARMSTON; LUZMILA ARROYO; NATALY ASCARRUNZ; CELSO PAULO DE AZEVEDO, CPAA; TIMOTHY BAKER; RADOMIR BALAZY; CAROLINE BEDEAU; NICHOLAS BERRY; ANDRII M. BILOUS; SVITLANA YU. BILOUS; PULCHÉRIE BISSIENGOU; LILIAN BLANC; KAPITOLINA S. BOBKOVA; TATYANA BRASLAVSKAYA; ROEL BRIENEN; DAVID F. R. P. BURSLEM; RICHARD CONDIT; AIDA CUNI-SANCHEZ; DILSHAD DANILINA; DENNIS DEL CASTILLO TORRES; GÉRALDINE DERROIRE; LAURENT DESCROIX; ELENEIDE DOFF SOTTA; MARCUS VINICIO NEVES D OLIVEIRA, CPAF-AC; CHRISTOPHER DRESEL; TERRY ERWIN; MIKHAIL D. EVDOKIMENKO; JAN FALCK; TED R. FELDPAUSCH; ERNEST G. FOLI; ROBIN FOSTER; STEFFEN FRITZ; ANTONIO DAMIAN GARCIA-ABRIL; ALEKSEY GORNOV; MARIA GORNOVA; ERNEST GOTHARD-BASSÉBÉ; SYLVIE GOURLET-FLEURY; MARCELINO CARNEIRO GUEDES, CPAF-AP; KEITH C. HAMER; FARIDA HERRY SUSANTY; NIRO HIGUCHI; EURÍDICE N. HONORIO CORONADO; WANNES HUBAU; STEPHEN HUBBELL; ULRIK ILSTEDT; VIKTOR V. IVANOV; MILTON KANASHIRO, CPATU; ANDERS KARLSSON; VIKTOR N. KARMINOV; TIMOTHY KILLEEN; JEAN-CLAUDE KONAN KOFFI; MARIA KONOVALOVA; FLORIAN KRAXNER; JAN KREJZA; HARUNI KRISNAWATI; LEONID V. KRIVOBOKOV; MIKHAIL A. KUZNETSOV; IVAN LAKYDA; PETRO I. LAKYDA; JUAN CARLOS LICONA; RICHARD M. LUCAS; NATALIA LUKINA; DANIEL LUSSETTI; YADVINDER MALHI; JOSÉ ANTONIO MANZANERA; BEATRIZ MARIMON; BEN HUR MARIMON JUNIOR; RODOLFO VASQUEZ MARTINEZ; OLGA V. MARTYNENKO; MAKSYM MATSALA; RAISA K. MATYASHUK; LUCAS JOSE MAZZEI DE FREITAS, CPATU; HERVÉ MEMIAGHE; CASIMIRO MENDOZA; ABEL MONTEAGUDO MENDOZA; OLGA V. MOROZIUK; LIUDMILA MUKHORTOVA; SAMSUDIN MUSA; DINA I. NAZIMOVA; TOSHINORI OKUDA; LUIS CLAUDIO DE OLIVEIRA, CPAF-AC; PETR V. ONTIKOV; ANDREY F. OSIPOV; STEPHAN PIETSCH; MAUREEN PLAYFAIR; JOHN POULSEN; VLADIMIR G. RADCHENKO; KENNETH RODNEY; ANDES H. ROZAK; ADEMIR ROBERTO RUSCHEL, CPATU; ERVAN RUTISHAUSER; LINDA SEE; MARIA SHCHEPASHCHENKO; NIKOLAY SHEVCHENKO; ANATOLY SHVIDENKO; MARCOS SILVEIRA; JAMES SINGH; BONAVENTURE SONKÉ; CINTIA RODRIGUES DE SOUZA, CPAA; KRZYSZTOF STERENCZAK; LEONID STONOZHENKO; MARTIN J. P. SULLIVAN; JUSTYNA SZATNIEWSKA; HERMANN TAEDOUMG; HANS TER STEEGE; ELENA TIKHONOVA; MARISOL TOLEDO; OLGA V. TREFILOVA; RUBEN VALBUENA; LUIS VALENZUELA GAMARRA; SERGEY VASILIEV; ESTELLA F. VEDROVA; SERGEY V. VERHOVETS; EDSON VIDAL; NADEZHDA A. VLADIMIROVA; JASON VLEMINCKX; VINCENT A. VOS; FOMA K. VOZMITEL; WOLFGANG WANEK; THALES A. P. WEST; HANNSJORG WOELL; JOHN T. WOODS; VERGINIA WORTEL; TOSHIHIRO YAMADA; ZAMAH SHARI NUR HAJAR; IRIÉ CASIMIR ZO-BI. |
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Título: |
The forest observation system, building a global reference dataset for remote sensing of forest biomass. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Scientific Data, v. 6, n. 198, p. 1-11, 2019. |
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DOI: |
https://doi.org/10.1038/s41597-019-0196-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Forest biomass is an essential indicator for monitoring the Earth?s ecosystems and climate. It is a critical input to greenhouse gas accounting, estimation of carbon losses and forest degradation, assessment of renewable energy potential, and for developing climate change mitigation policies such as REDD+, among others. |
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Thesagro: |
Biomassa; Sensoriamento Remoto. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/202898/1/Nature-Schepaschenko-Biomassa.pdf
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Marc: |
LEADER 05124naa a2201861 a 4500
001 2113119
005 2019-10-16
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41597-019-0196-1$2DOI
100 1 $aSCHEPASCHENKO, D.
245 $aThe forest observation system, building a global reference dataset for remote sensing of forest biomass.$h[electronic resource]
260 $c2019
520 $aForest biomass is an essential indicator for monitoring the Earth?s ecosystems and climate. It is a critical input to greenhouse gas accounting, estimation of carbon losses and forest degradation, assessment of renewable energy potential, and for developing climate change mitigation policies such as REDD+, among others.
650 $aBiomassa
650 $aSensoriamento Remoto
700 1 $aCHAVE, J.
700 1 $aPHILLIPS, O. L.
700 1 $aLEWIS, S. L.
700 1 $aDAVIES, S. J.
700 1 $aRÉJOU-MÉCHAIN, M.
700 1 $aSIST, P.
700 1 $aSCIPAL, K.
700 1 $aPERGER, C.
700 1 $aHERAULT, B.
700 1 $aLABRIÈRE, N.
700 1 $aHOFHANSL, F.
700 1 $aAFFUM-BAFFOE, K.
700 1 $aALEINIKOV, A.
700 1 $aALONSO, A.
700 1 $aAMANI, C.
700 1 $aARAUJO-MURAKAMI, A.
700 1 $aARMSTON, J.
700 1 $aARROYO, L.
700 1 $aASCARRUNZ, N.
700 1 $aAZEVEDO, C. P. de
700 1 $aBAKER, T.
700 1 $aBALAZY, R.
700 1 $aBEDEAU, C.
700 1 $aBERRY, N.
700 1 $aBILOUS, A. M.
700 1 $aBILOUS, S. Y.
700 1 $aBISSIENGOU, P.
700 1 $aBLANC, L.
700 1 $aBOBKOVA, K. S.
700 1 $aBRASLAVSKAYA, T.
700 1 $aBRIENEN, R.
700 1 $aBURSLEM, D. F. R. P.
700 1 $aCONDIT, R.
700 1 $aCUNI-SANCHEZ, A.
700 1 $aDANILINA, D.
700 1 $aTORRES, D. del C.
700 1 $aDERROIRE, G.
700 1 $aDESCROIX, L.
700 1 $aSOTTA, E. D.
700 1 $aOLIVEIRA, M. V. N. d'
700 1 $aDRESEL, C.
700 1 $aERWIN, T.
700 1 $aEVDOKIMENKO, M. D.
700 1 $aFALCK, J.
700 1 $aFELDPAUSCH, T. R.
700 1 $aFOLI, E. G.
700 1 $aFOSTER, R.
700 1 $aFRITZ, S.
700 1 $aGARCIA-ABRIL, A. D.
700 1 $aGORNOV, A.
700 1 $aGORNOVA, M.
700 1 $aGOTHARD-BASSÉBÉ, E.
700 1 $aGOURLET-FLEURY, S.
700 1 $aGUEDES, M. C.
700 1 $aHAMER, K. C.
700 1 $aSUSANTY, F. H.
700 1 $aHIGUCHI, N.
700 1 $aCORONADO, E. N. H.
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700 1 $aHUBBELL, S.
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700 1 $aKONOVALOVA, M.
700 1 $aKRAXNER, F.
700 1 $aKREJZA, J.
700 1 $aKRISNAWATI, H.
700 1 $aKRIVOBOKOV, L. V.
700 1 $aKUZNETSOV, M. A.
700 1 $aLAKYDA, I.
700 1 $aLAKYDA, P. I.
700 1 $aLICONA, J. C.
700 1 $aLUCAS, R. M.
700 1 $aLUKINA, N.
700 1 $aLUSSETTI, D.
700 1 $aMALHI, Y.
700 1 $aMANZANERA, J. A.
700 1 $aMARIMON, B.
700 1 $aMARIMON JUNIOR, B. H.
700 1 $aVASQUEZ MARTINEZ, R.
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700 1 $aMATYASHUK, R. K.
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700 1 $aMEMIAGHE, H.
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700 1 $aMONTEAGUDO MENDONZA, A.
700 1 $aMOROZIUK, O. V.
700 1 $aMUKHORTOVA, L.
700 1 $aMUSA, S.
700 1 $aNAZIMOVA, D. I.
700 1 $aOKUDA, T.
700 1 $aOLIVEIRA, L. C. de
700 1 $aONTIKOV, P. V.
700 1 $aOSIPOV, A. F.
700 1 $aPIETSCH, S.
700 1 $aPLAYFAIR, M.
700 1 $aPOULSEN, J.
700 1 $aRADCHENKO, V. G.
700 1 $aRODNEY, K.
700 1 $aROZAK, A. H.
700 1 $aRUSCHEL, A. R.
700 1 $aRUTISHAUSER, E.
700 1 $aSEE, L.
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700 1 $aSHEVCHENKO, N.
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700 1 $aVALENZUELA GAMARRA, L.
700 1 $aVASILIEV, S.
700 1 $aVEDROVA, E. F.
700 1 $aVERHOVETS, S. V.
700 1 $aVIDAL, E.
700 1 $aVLADIMIROVA, N. A.
700 1 $aVLEMINCKX, J.
700 1 $aVOS, V. A.
700 1 $aVOZMITEL, F. K.
700 1 $aWANEK, W.
700 1 $aWEST, T. A. P.
700 1 $aWOELL, H.
700 1 $aWOODS, J. T.
700 1 $aWORTEL, V.
700 1 $aYAMADA, T.
700 1 $aHAJAR, Z. S. N.
700 1 $aZO-BI, I. C.
773 $tScientific Data$gv. 6, n. 198, p. 1-11, 2019.
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Embrapa Amazônia Ocidental (CPAA) |
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